Illuminating the Path for Super-resolution Imaging with Improved Rhodamine Dyes

SUTD: Chao Wang, Weijie Chi, Xiaogang Liu
DUT: Zhiwei Ye, Wei Yang, Ying Zheng, Zhenlong Huang, Xiaoyuan Li, Yi Xiao

DUT and SUTD researchers developed a new strategy that enhances the brightness and clarity of sub-cellular structures when dyed with novel rhodamine fluorophores, laying the ground for the advancement of super-resolution microscopes.

Researchers from DUT and SUTD developed a new class of quaternary piperazine-substituted rhodamines with outstanding quantum yields (Φ = 0.93) and superior brightness (ε × Φ = 8.1 × 104 L·mol–1·cm–1), for imaging cell membranes and lysosomes in biological cells with super-resolution microscopy.

Recent years have witnessed a rapid evolution of advanced fluorescence imaging techniques, such as single-molecule localization microscopy (SMLM) that allows for unprecedented resolution beyond the Abbe diffraction limit of the optical microscope.

You can find more details at https://www.sutd.edu.sg/Research/Research-News/2019/12/Illuminating-the-path-for-super-resolution-imaging